250 milliliter bottle of Proteomic Stabilizer PROT1. Sufficient to stabilize over 170 milliliters of whole blood when used as recommended. Also includes buffer used after thawing samples stored frozen in Stabilizer: THAWLYSE1 "Thaw-Lyse, 1000X Concentrate".
Fisher Scientific also carries this product for Smart Tube Inc. and lists it as Part Number: 501351692
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Protocol for banking samples with PROT1 |
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Protocol for thawing samples banked with PROT1 |
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Published antibody panels
used to analyze samples banked in PROT1
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| Published Protocols and Methods Papers using PROT1 |
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Smets, Tina, et al. "Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF)." Multiple Myeloma. Humana Press, New York, NY, 2018. 47-54. |
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Behbehani, Gregory K. "Cell Cycle Analysis by Mass Cytometry." Cellular Quiescence. Humana Press, New York, NY, 2018. 105-124. |
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Fernandez, Rosemary, and Holden Maecker. "Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass Cytometry." Bio-protocol 5.11 (2015). |
See our Publications page for selected studies that used this buffer to bank their samples as well as download tables of the antibodies they used to analyze the samples.
Publications that used this buffer to bank their samples
Geanon, Daniel, et al. "A Streamlined CyTOF Workflow To Facilitate Standardized Multi-Site Immune Profiling of COVID-19 Patients." medRxiv 2020.06.26.20141341 (2020).
Rahil, Zainab, et al. "Landscape of coordinated immune responses to H1N1 challenge in humans." Journal of Clinical Investigation, Vol. 130 (2020).
Brandt, Raphael, et al. "Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium." Nature Communications, Vol. 10, Article 2919 (2019).
Tsai, Amy, et al. "A year-long immune profile of the systemic response in acute stroke survivors." Brain, Vol. 142, Iss. 4 (2019).
Chen, Luxi, et al. "CD56 Expression Marks Human Group 2 Innate Lymphoid Cell Divergence from a Shared NK Cell and Group 3 Innate Lymphoid Cell Developmental Pathway." Immunity (2018).
Ajami, Bahareh, et al. "Single-cell mass cytometry reveals distinct populations of brain myeloid cells in mouse neuroinflammation and neurodegeneration models." Nature Neuroscience 21.4 (2018): 541.
Spitzer, Matthew H., et al. "Systemic immunity is required for effective cancer immunotherapy." Cell 168.3 (2017): 487-502.
Glassberg, Jeffrey, et al. "Application of phospho-CyTOF to characterize immune activation in patients with sickle cell disease in an ex vivo model of thrombosis." Journal of immunological methods 453 (2018): 11-19.
Blazkova, Jana, et al. "Multicenter systems analysis of human blood reveals immature neutrophils in males and during pregnancy." The Journal of Immunology (2017): 1601855.
Schulz, Axel Ronald, et al. "Silver nanoparticles for the detection of cell surface antigens in mass cytometry." Cytometry Part A 91.1 (2017): 25-33.
Ciarlo, Eleonora, et al. "Impact of the microbial derived short chain fatty acid propionate on host susceptibility to bacterial and fungal infections in vivo." Scientific reports 6 (2016): 37944.
Fragiadakis, Gabriela K., et al. "Patient-specific immune states before surgery are strong correlates of surgical recovery." Anesthesiology: The Journal of the American Society of Anesthesiologists 123.6 (2015): 1241-1255.
Spitzer, Matthew H., et al. "An interactive reference framework for modeling a dynamic immune system." Science 349.6244 (2015): 1259425.
Behbehani, Gregory K., et al. "Mass cytometric functional profiling of acute myeloid leukemia defines cell cycle and immunophenotypic properties that correlate with known responses to therapy." Cancer discovery (2015): CD-15.
Gaudillière, Brice, et al. "Implementing mass cytometry at the bedside to study the immunological basis of human diseases: distinctive immune features in patients with a history of term or preterm birth." Cytometry Part A 87.9 (2015): 817-829.
Gaudilliere, Brice, et al. "Coordinated surgical immune signatures contain correlates of clinical recovery." Science translational medicine 6.255 (2014): 255ra131.
Behbehani, Gregory K., et al. "Single-cell mass cytometry adapted to measurements of the cell cycle." Cytometry Part A 81.7 (2012): 552-566.
